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Preparation of the lysozyme–DSM samples for SAXS. Dry DSM particles are suspended in pure water. After swelling, dissolved lysozyme is added, and the mixture is incubated under rotation. The DSM particles with absorbed lysozyme are isolated by filtering the mixture, removing external liquid. The particles were subsequently transferred to a glass capillary for SAXS.

Journal: Molecular Pharmaceutics

Article Title: A Structural Study on Absorption of Lysozyme in Amorphous Starch Microspheres

doi: 10.1021/acs.molpharmaceut.4c00135

Figure Lengend Snippet: Preparation of the lysozyme–DSM samples for SAXS. Dry DSM particles are suspended in pure water. After swelling, dissolved lysozyme is added, and the mixture is incubated under rotation. The DSM particles with absorbed lysozyme are isolated by filtering the mixture, removing external liquid. The particles were subsequently transferred to a glass capillary for SAXS.

Article Snippet: The filtered particles were transferred to 1.0–1.5 mm thick glass capillaries from Hilgenberg (Malsfeld, Germany.

Techniques: Incubation, Isolation

Scattering from DSM with and without lysozyme. (A, B) Microscope image of DSM particles (average swollen diameter: 400 μm) in the hydrated state used in the SAXS experiments, calculated to be 20 wt % DSM. Panel A shows the particles imaged on glass slides, showing a morphology consistent with what has previously been reported. Panel B shows the particles imaged in a SAXS glass capillary, where a crammed environment deforms the particles slightly. (C, D) SAXS data of DSM with or without absorbed lysozyme. In panel C, DSM particles of an average swollen diameter of 400 μm were used, while 580 μm was the average diameter of the particles used in panel D. Arrows in panels C and D point to the bump that appears in the presence of lysozyme, coming from the form factor of the protein.

Journal: Molecular Pharmaceutics

Article Title: A Structural Study on Absorption of Lysozyme in Amorphous Starch Microspheres

doi: 10.1021/acs.molpharmaceut.4c00135

Figure Lengend Snippet: Scattering from DSM with and without lysozyme. (A, B) Microscope image of DSM particles (average swollen diameter: 400 μm) in the hydrated state used in the SAXS experiments, calculated to be 20 wt % DSM. Panel A shows the particles imaged on glass slides, showing a morphology consistent with what has previously been reported. Panel B shows the particles imaged in a SAXS glass capillary, where a crammed environment deforms the particles slightly. (C, D) SAXS data of DSM with or without absorbed lysozyme. In panel C, DSM particles of an average swollen diameter of 400 μm were used, while 580 μm was the average diameter of the particles used in panel D. Arrows in panels C and D point to the bump that appears in the presence of lysozyme, coming from the form factor of the protein.

Article Snippet: The filtered particles were transferred to 1.0–1.5 mm thick glass capillaries from Hilgenberg (Malsfeld, Germany.

Techniques: Microscopy

Materials

Journal: Journal of visualized experiments : JoVE

Article Title: Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes

doi: 10.3791/64439

Figure Lengend Snippet: Materials

Article Snippet: 4 IN thinwall GL 1.0 OD/.75 ID , World precision instrument , TW100F-4 , Injection needles.

Techniques: Injection, Microscopy, Imaging